Table 1 Sequences of primers and siRNAs used in the study.
Primer/siRNA | Sequence (5’-3’) |
|---|---|
PRRSV ORF7-F | AAACCAGTCCAGAGGCAAG |
PRRSV ORF7-R | TCAGTCGCAAGAGGGAAAT |
monkey HPSE-F | CTTCGTACCTTGGCCAGAGG |
monkey HPSE-R | CTTCTCCACCAGCCTTCAGG |
monkey HSPG2-F | CCTGACGGCCACTTCTACC |
monkey HSPG2-R | GCAGGCATCACCACATTCAC |
monkey Sdc-4-F | CACTGAAACCAAGAAACTG |
monkey Sdc-4-R | GTTAGACACATCCTCACTT |
monkey CD163-F | TTCACTGCACTGGGACTGAG |
monkey CD163-R | AGGACAGTGTTTGGGACTGG |
monkey GAPDH-F | CCTTCCGTGTCCCTACTGCCAA |
monkey GAPDH-R | GACGCCTGCTTCACCACCTTCT |
pig β-actin-F | CTCCATCATGAAGTGCGACGT |
pig β-actin-R | GTGATCTCCTTCTGCATCCTGTC |
DHODH-F _ EcoRI | CGAATTCATGGCGTGGAGACA |
DHODH-R _ XhoI | CGCTCGAGTCAAGCGTAATCTGGAACATCGTATGGGTACCTCCGATGATCTGC |
siRNA-1-sense | AAGCCGUGGACGGACUUUAUAdTdT |
siRNA-1-antisense | UAUAAAGUCCGUCCACGGCUUdTdT |
siRNA-2-sense | GGUAUGGAUUUAACAGUCACGdTdT |
siRNA-2-antisense | UGACUGUUAAAUCCAUACCUGdTdT |
siRNA-3-sense | CGGGAUUUAUCAACUCAAACCdTdT |
siRNA-3-antisense | UUUGAGUUGAUAAAUCCCGGAdTdT |
siNC-sense | UUCUCCGAACGUGUCACGUdTdT |
siNC-antisense | ACGUGACACGUUCGGAGAAdTdT |