Figure 4
Time-of-addition analysis of vidofludimus anti-PRRSV activity. A Schematic illustration of the time-of-addition experiment. DMSO was used as the solvent of Vi. And an equal volume of DMSO was used for treating viruses as black solvent control. B TCID50 detection for virucidal activity assay. C PRRSV ORF7 mRNA levels determination by qRT-PCR in virus binding assay. GAPDH was used as reference control. D PRRSV ORF7 mRNA levels determination by qRT-PCR in virus internalization assay. GAPDH was used as reference control. E PRRSV ORF7 mRNA levels determination by qRT-PCR in virus replication assay. GAPDH was used as reference control. F Virus titration by TCID50 calculation with cell supernatant in virus release assay. G PRRSV ORF7 mRNA levels determination by qRT-PCR in virus binding assay in PAMs. β-actin was used as reference control. H PRRSV ORF7 mRNA levels determination by qRT-PCR in virus replication assay in PAMs. β-actin was used as reference control. Error bars represent the mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns: not significant.