Figure 1

High-throughput screening (HTS) for inhibitors of PRRSV infection from an FDA-approved drug library. A HTS assay timeline. B HTS assay flowchart. C Each dot represents the percent inhibition of PRRSV (0.01 MOI) achieved with by compound (5 μM). D IFA of infected Marc-145 cells treated with one of the four designated compounds. PRRSV N protein is colored green, and brightfield-imaged cells show CPE. Scale bars, 500 μm. E CC₅₀ and EC₅₀ of Ta (i), Vi (ii), Be (iii) and Co (iv). Insets show the structure of each compound.