Table 1 Antibodies used in this study
Primary antibodies | Clone | Isotype | Working dilution | Supplier |
|---|---|---|---|---|
FITC-ASFV major capsid protein p72 | 1BC11 | FITC-conjugated | 1:100 | Ingenasa |
Porcine CD163 | 2A10 | IgG1 | 1:200 | Bio-Rad |
Human CD163 | Polyclonal | 1:200 | R&D systems | |
Porcine CD14 | MIL2 | IgG2b | 1:100 | [21] |
Porcine SWC3 (CD172a) | DH59B | IgG1 | 1:50 | VMRD |
Porcine Sn | 41D3 | IgG1 | 1:50 | [22] |
Human CD1c | L161 | IgG1 | 1:50 | Biolegend |
Porcine MHCII | MSA3 | IgG2a | 1:200 | Kingfisher Biotech |
Human cytokeratin | AE1/AE3 | IgG1 | 1:50 | Dako |
Porcine vimentin | V9 | IgG1 | 1:50 | Bio-Rad |
Human vWF | Polyclonal | 1:50 | Dako |
Secondary antibodies (conjugate) | Host/species reactivity | Isotype | Working dilution | Supplier |
|---|---|---|---|---|
Alexa Fluor 594 | Goat anti-mouse | IgG1 | 1:400 | Invitrogen |
Alexa Fluor 594 | Goat anti-mouse | IgG2a | 1:500 | Invitrogen |
Alexa Fluor 594 | Goat anti-mouse | IgG2b | 1:200 | Invitrogen |
Alexa Fluor 594 | Rabbit anti-goat | IgG (H+L) | 1:200 | Invitrogen |
Texas Red | Goat anti-rabbit | IgG (H+L) | 1:50 | Invitrogen |
Alexa Fluor 647 | Rabbit anti-goat | IgG (H+L) | 1:300 | Invitrogen |