Figure 3

Characterization of the ASFV infected cells in different areas of the nasal explants. A Double immunofluorescence staining of ASFV (green) together with different cell type markers (red) was performed on the nasal explant. Images were taken from the explants at 72 hpi. Double positive cells are indicated with white arrows. Scale bar: 50 μm. B Percentage of cell marker-positive cells within the ASFV-infected cells in each two nasal tissue type inoculated with E70 and BEL18 strains. Statistical significance was determined by two-way ANOVA followed by Tukey’s multiple comparison post hoc test. Different letters represent significant differences (p < 0.05) of double-positive cells in different areas of the nasal mucosa: epithelium (EP), lamina propria (LP), and submucosa (SM). All data are presented as mean value of three animals ± SD.