Streptococcus ruminantium-associated sheep mastitis outbreak detected in Italy is distinct from bovine isolates

Table 1 Oligonucleotide sequences used in this study.

Target gene	Primer name	Primer sequence (5ʹ—3ʹ)	T_A (°C)	Amplicon size (bp)	References
recN	SSrecN-F SSrecN-R	CTACAAACAGCTCTCTTCT ACAACAGCCAATTCATGGCGTGATT	60	336	[7]
gdh	JP4 JP5	GCAGCGTATTCTGTCAAACG CCATGGACAGATAAAGATGG	55	688	[10]
16S rRNA	F R	GCAAGTGGAACGCAACTTTTCA CTATGTATCGTTGCCTTGGTAG	60	240	[11]
gap	Strept-gap-F Strept-gap-R	ACTCAAGTGTACGAACAAGT GTCTTGCATTCCGTCGTAT	54	945	[16]

ISSN: 1297-9716