Figure 7

SC suppresses the activation of NF-κB to inhibit PRRSV infection. A, B Marc-145 cells were seeded in 12-well plates and cultured for 12 h. The cells were infected with PRRSV SD-YL1712 (MOI = 1), and cells were collected at 24 and 36 hpi for Western blotting to determine the expression levels of P65, p-P65, IκBα, and p-IκBα. C–E Marc-145 cells in 12-well plates were infected with PRRSV SD-YL1712 (MOI = 1) and then treated with 10 µM SC. Cells were harvested at 36 hpi for Western blotting to determine the expression levels of P65, p-P65, IκBα, p-IκBα, and PRRSV N. F, G Marc-145 cells were seeded in 12-well plates, cultured for 12 h, infected with PRRSV SD-YL1712 (MOI = 1), and then treated with 2 µM Bay11-7082. At 36 hpi, cells were collected and analysed to determine the expression levels of P65, p-P65, IκBα, p-IκBα, and N by Western blotting.