Figure 6

SC inhibits the replication of different PRRSV strains and multiple other viruses. A Marc-145 cells were seeded in 12-well plates, cultured for 12 h, and infected with different PRRSV strains (SD-YL1712, VR-2332, CH-1a, JXA1, NADC30, and type 1 PRRSV GZ11). Thirty-six hours after PRRSV infection, cell samples were collected to measure the PRRSV ORF7 expression level. B Forty-eight hours after treatment with 10 µM SC, the toxicity of 10 µM SC to PAMs, Vero cells, and PK-15 cells was detected by CCK-8 assay. PBS was used as a negative control, and the cell viability in this group was set to 100%. C, D Vero cells were infected with PEDV, and PK-15 cells were infected with PCV2, CSFV, and PRV (MOI = 1). Cells were harvested at 12, 24, and 36 hpi to determine the expression levels of viral genes and viral proteins. *P < 0.05; **P < 0.01; ***P < 0.001 compared with the respective virus control.