Figure 4

SC does not interact with PRRSV directly. A–C Schematic diagram of the interaction between SC and PRRSV. Ten microlitres of 1 mM SC solution was added to 990 µL of PRRSV original solution. Ten microlitres of DMSO was added to the negative control group, and 10 µL of neutralization antibody was added to the positive control group. After incubation at 4 °C for 1 h, Marc-145 cells were infected with 2 µL of the virus mixture, and supernatant and cell samples were collected at 36 and 48 hpi to measure PRRSV N expression levels and virus titres. D, E Marc-145 cells were infected with PRRSV at MOIs ranging from 1 to 30, and supernatant and cell samples were collected at 36 hpi to detect the virus titre and PRRSV N expression level. *P < 0.05; **P < 0.01; ***P < 0.001 compared with the respective virus control.