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Figure 3 | Veterinary Research

Figure 3

From: The natural compound Sanggenon C inhibits PRRSV infection by regulating the TRAF2/NF-κB signalling pathway

Figure 3

SC suppresses PRRSV infection and inflammatory cytokine expression in PAMs. A–C PAMs were seeded in 12-well plates, cultured for 6 h, infected with PRRSV SD-YL1712 (MOI = 1), and then treated with different concentrations of SC. Supernatants and cells were harvested at 24 hpi to determine PRRSV ORF7 expression, virus titre and PRRSV N expression. D PAMs were seeded in 96-well plates, cultured for 6 h, infected with GFP-PRRSV (MOI = 1), and treated with different concentrations of SC. After 24 h of GFP-PRRSV infection, the fluorescence of GFP was measured with a Nikon fluorescence microscope. E PAMs were seeded in 12-well plates, cultured for 6 h, infected with PRRSV SD-YL1712 (MOI = 1), and then treated with 10 µM SC. Cells were harvested at 24 hpi to measure inflammatory cytokine levels. *P < 0.05; **P < 0.01; ***P < 0.001 compared with the respective virus control.

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Veterinary Research

ISSN: 1297-9716