Table 2 Plasmids construction in this study.
From: Characterization of translocon proteins in the type III secretion system of Lawsonia intracellularis
Plasmids | Genotype/description |
|---|---|
pBAD24-LI1157-HA | araC promoter, LI1157-HA, ampicillin |
pBAD24-LI1158-HA | araC promoter, LI1158-HA, ampicillin |
pBAD24-LI1159-HA | araC promoter, LI1159-HA, ampicillin |
pBAD24-LI1158N40-YopEΔ15-HA | araC promoter, LI1158N40-YopEΔ15-HA, ampicillin |
pBAD24-LI1159N40-YopEΔ15-HA | araC promoter, LI1159N40-YopEΔ15-HA, ampicillin |
pRS416-GAL1-LI1158 | Gal1 promoter, 3 × Flag-LI1158, ampicillin |
pSIN-EF1-puro-SFB-LI1158 | EF1α promoter, LI1158-S-tag-2 × Flag-SBP, ampicillin |
pGADT7-LI1157 | T7 promoter, AD-HA-LI1157, ampicillin |
pGADT7-LI1158 | T7 promoter, AD-HA-LI1158, ampicillin |
pGADT7-LI1159 | T7 promoter, AD-HA-LI1159, ampicillin |
pGBKT7-LI1158 | T7 promoter, BD-c-Myc-LI1158, Kanamycin |
pGBKT7-LI1159 | T7 promoter, BD-c-Myc-LI1159, Kanamycin |