Table 1 Primers used in this study.
From: Characterization of translocon proteins in the type III secretion system of Lawsonia intracellularis
Primer | Sequence 5′–3′ (restriction enzyme sites are underlined) |
|---|---|
pBAD24-LI1157-F | GTTTTTTTGGGCTAGCAGGAGGAATTCATGGTTATGAAACAAGAG |
pBAD24-LI1157-R | ATCTGGTACGTCGTATGGGTAGTCGACTTGCTTTACTCCTCCAGA |
pBAD24-LI1158-F | GTTTTTTTGGGCTAGCAGGAGGAATTCATGACAAATTTTGGAGAT |
pBAD24-LI1158-R | ATCTGGTACGTCGTATGGGTAGTCGACCTCACGTGCACCACGTTG |
pBAD24-LI1158N40-R | GCTAGATCCTGACACAGAACCACCTTGCTCTTTAGG |
pBAD24-LI1159-F | GTTTTTTTGGGCTAGCAGGAGGAATTCATTTTGGAGAAGACTATC |
pBAD24-LI1159-R | ATCTGGTACGTCGTATGGGTAGTCGACCGCCAGAATACGCTGTGT |
pBAD24-LI1159N40-R | CGCTGCTAGATCCTGACACAGATTTAGCAGGTTGTCCACCAGAGGA |
pRS416-GAL1-LI1158-F | ATTACAAGGATGACGATGACAATGGCGGAGGAGCGGCCGCGATGACAAATTTTGGAGAT |
pRS416-GAL1-LI1158-R | GCGTGAATGTAAGCGTGACATAACTAATTACATGACTCGAGTTACTCACGTGCACCACG |
pSIN-LI1158-F | GGATCCCCGGACGAATTCTTCGAAACCATGACAAATTTTGGAGAT |
pSIN-LI1158-R | AGCAGCAGCGGTTTCTTTGCTAGC CTCACGTGCACCACGTTG |
pGADT7-LI1157-F | CCGGAATTCATGGTTATGAAACAAGAGC |
pGADT7-LI1157-R | CCCTCGAGTTGCTTTACTCCTCCAGAGAGAAT |
pGADT7-LI1158-F | GCCATGGAGGCCAGTGAATTCATGACAAATTTTGGAGATATAAG |
pGADT7-LI1158-R | ACGATTCATCTGCAGCTCGAGTTACTCACGTGCACCACG |
pGBKT7-LI1158-F | CATATGGCCATGGAGGCCGAATTCACAAATTTTGGAGATATA |
pGBKT7-LI1158-R | TGCGGCCGCTGCAGGTCGACGGATCCCTTACTCACGTGCACCACG |
pGADT7-LI1159-F | CCGGAATTCATTTTGGAGAAGACTATCA |
pGADT7-LI1159-R | CCCTCGAGCGCCAGAATACGCTGTGTTGTTGC |
pGBKT7-LI1159-F | CATATGGCCATGGAGGCCGAATTCATTTTGGAGAAGACTATC |
pGBKT7-LI1159-R | CGGCCGCTGCAGGTCGACGGATCCTTACGCCAGAATACGCTG |