Figure 5
From: Characterization of translocon proteins in the type III secretion system of Lawsonia intracellularis
LI1158 and LI1159 self-interact and LI1159 interacts with LI1157. A–C Different combinations of plasmids (bait and prey) were transformed into yeast strain Y2H Gold, and then streaked on selective medium (Leu-, Trp-; growth control) and low stringency medium (Leu-, Trp-, His-), respectively. The plasmids pGBKT7-p53 (human protein p53) and pGADT7-T (large Tantigen of SV40) were co-transformed into yeast cells as positive control. Then, self-activation was carried out to overrule the interaction with vectors.