Figure 5

Histopathological section of ovine liver parasitized with Fasciola hepatica shows different levels of leukocyte infiltration. A multifocal infiltration of inflammatory cells especially eosinophils (yellow boxes) along with fibrotic areas (yellow arrow) in proximity to branch of bile ducts. B predominant infiltration of macrophages and PMN in proximity to bile ducts. C moderate infiltration of inflammatory cells especially plasma cells and lymphocytes in the portal area. All images: Hematoxylin & Eosin (H&E) staining, ×400 magnification. Immunofluorescence analysis on consecutive sections used for H&E staining. DNA (D, H; blue), pan-histones (H1, H2A/H2B, H3, H4) (E, I; red) and neutrophil elastase (NE) (F, J; green). Depiction of the merge of the three channels (G 63X magnification, K 40X magnification). 3D imaging video on immunofluorescence analysis of tissue sections provided in Additional file 3.