Figure 3

Fasciola hepatica antigen (FhAg)-stimulated ovine PMN increases intracellular ROS production by oxidation of 2′,7′-dichlorofluorescein diacetate to fluorescent DCF. Ovine PMN (n = 3) were incubated with FhAg (10 or 100 μg/mL), in duplicates for 90 min. As controls, plain medium (negative control) and zymosan (1 mg/mL; positive control) were used. A Significant differences on ROS production, negative control and FhAg-stimulated PMN (two tailed t-test p = 0.0286). Each dot represents the average/mean of values at 90 min B Determination of intracellular ROS production by 90-min temporal analysis was measured via spectrophotometry analysis by using an automated multiplate reader. In both, a clear dose dependent pattern is observed.