Figure 3
From: Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie
The atypical scrapie inoculum PS152 shows a C‑BSE signature after propagation in PMCA using TgBov substrate. PMCA reaction products from the PS152 inoculum (PMCA-PS152) were subjected to PrPSc detection by western blot. Positive PMCA reactions from cattle C-BSE (PMCA-BSE + C) and scrapie (PMCA-Scrapie C+) propagated in tgBov substrate were used as controls. A classical scrapie isolate (Scrapie + C) and a C-BSE isolate (BSE + C) were used as positive controls for western blot. Using the Sha31 antibody, PS152 PMCA products displayed an indistinguishable glycoform pattern to that of cattle C-BSE and cattle C-BSE propagated by PMCA.