Figure 1
From: Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie
No PrPSc accumulation by western Blot is detected in brains from cows intracerebrally challenged with atypical scrapie. Frontal cortex (Fc), thalamus (Th), cerebellum (Cbl) and obex homogenates from the four atypical scrapie-challenged cows and a negative control cow were subjected to PrPSc detection by western blot using the Sha31 antibody. No positivity was detected in any of the experimentally inoculated cows. Brain homogenates from a classical scrapie-infected sheep (Scrapie + C) and a BSE-infected cow (BSE + C) were used as positive controls.