Experimental transmission of ovine atypical scrapie to cattle

Table 1 Cattle genotypes, survival times and cause of cull

Group	Animal	Inoculum	ORF	23 bp	12 bp	Survival time [mpi]	Cause of cull
2	92/20	AHQ/AHQ brain	6:6 Q78 het	+/−	+/−	106	Timed cull
	54/20	AHQ/AHQ brain	6:6	−/−	−/−	106	Timed cull
	1/21	None	6:6	−/−	−/−	112	Downer cow (injury)
1	2/21	AFRQ/AFRQ brain	6:6 Q78 hom	+/−	+/−	106	Timed cull
	2/20	AFRQ/AFRQ brain	6:6 Q78 het N192 het	+/−	+/−	91	Tumour (soft tissue sarcoma)
	6/17	AFRQ/AFRQ brain	6:6 Q78 hom	+/+	+/+	48	TSE suspect
	6/16	AFRQ/AFRQ brain	6:6 Q78 hom	+/+	+/+	46	Bovine tuberculosis^a
	4/15	AFRQ/AFRQ brain	6:6 Q78 hom	+/+	−/−	23	Hip fracture
	1/20	Saline	6:5	−/−	+/−	94	Downer cow (foot abscess)
	55/20	Saline	6:6 Q78 het	+/−	+/−	91	Timed cull
	17/17	Saline	6:6 Q78 het	+/−	+/−	51	Timed cull
	8/17	Saline	6:5	−/−	+/−	50	Timed cull
	14/15	Saline	6:6 Q78 hom	+/+	+/+	33	Timed cull

ORF: open reading frame of the bovine PrP gene detailing the number of N-terminal octapeptide repeats, the silent polymorphisms Q78 and N192, either homozygous (hom) or heterozygous (het) at position 78 and 192 of the ORF respectively compared to the wild type; and the 23 and 12 bp indels (− deletion allele, + insertion allele) of the promoter PrP gene, mpi: months post-inoculation or post-test group inoculation for 1/21, rounded down to the nearest month. The TSE suspect is highlighted in bold letters.
^aThis case has been reported separately [56].

ISSN: 1297-9716