Figure 5
From: Experimental transmission of ovine atypical scrapie to cattle
Thermostability analysis of the positive PMCA products. A (left) Schematic representation of the PMCA results after heat treatment. The results obtained for each inoculum are represented by colored squares. Darker color relates to positives tubes. Positive controls: Bovine BSE. Negative controls: classical scrapie thermosensitive control (PS21 thermosensitive control), non-inoculated Bov-Tg110 substrate (none). B (right) Western immunoblot analysis of the PMCA products after heat treatment. PrPres signature is BSE after PMCA amplification in Bov-Tg110 substrate following heat treatment proving thermostability. Only the thermosensitive control failed to amplify. Sha31 1/5000. Molecular mass markers in kilodaltons (kDa) are indicated on the right side of the blots.