Figure 3
From: Experimental transmission of ovine atypical scrapie to cattle
Schematic representation of the PMCA results of cattle samples. The results obtained for the 7 rounds of amplification for each inoculum are represented by colored squares. Darker color relates to higher number of positives tubes within triplicates. Positive controls: Cattle BSE inocula adapted to PMCA amplification in Bov-Tg110 brain (PMCA adapted bovine BSE), Bov-Tg110 mice inoculated with atypical scrapie but negative for PrPres detection (ovine atypical scrapie 1/Tg110 and ovine atypical scrapie 2/Tg110; these controls were added to mimic the steer situation after challenge with atypical scrapie). Negative controls: non-inoculated Bov-Tg110 substrate (none). Bovine BSE PMCA amplification titration in Bov-Tg110 substrate (bovine BSE, bovine BSE 10−3, 10−7 and 10−10).