Figure 8

Effects of the PB2 S155N and PA S49Y and D347G mutations on viral polymerase activity in 293t and DF-1 cells. 293T cells (A) or DF-1 cells (B) were transfected with rJS/875 wild-type or mutant RNP complexes composed of PB2 WT or PB2 S155, PB1 WT, PA WT or PA S49Y, PA D347G and NP WT protein expression plasmids, together with the firefly luciferase reporter and a Renilla luciferase reporter (internal control). Luciferase activity was measured at 48 h post-transfection at 33 ℃ or 37 ℃. The data presented are the mean ± SD of three independent experiments. P values were calculated by using the Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.