Figure 10

The growth rate of rJS/875 and rJS/875-MA in mixed infection. The rJS/875 and rJS/875-MA (MA#12) viruses were mixed at a 1:1 ratio (P0) to infect mice (n = 3), and on day 3 after infection, the mice were euthanized, and the supernatant of mouse lung tissue was collected (P1) and used to infect the next three mice (P2). P0, P1 and P2 viral fluids were collected, RNA extraction, fragment amplification and sequencing were performed, and the peaks of the PB2 S155N and PA S49Y and D347G positions were observed using Chromas software.