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Table 3 List of MOK124 (CC151) candidate immunogenic cell wall-anchored proteins detected by mass spectrometry.

From: Immunoproteomic analysis of the serum IgG response to cell wall-associated proteins of Staphylococcus aureus strains belonging to CC97 and CC151

Serum source

Spot number

Protein name

# Unique peptides

Predicted localisation

Theoretical Mr (kDa)/pI *

Observed Mr (kDa)/pI +

Cow 504 (MOK124 infected)

1

Clumping factor B (ClfB)

9

Cell wall

92/4.2

 ~120/4.8–5

2

Bone sialoprotein-binding protein (Bbp)

6

Cell wall

121/4.55

 ~80/4.2–4.5

10

Iron regulated surface determinant protein A (IsdA)

2

Cell wall

39/9.6

 ~35/8.1–8.6

11

Iron regulated surface determinant protein A (IsdA)

2

Cell wall

39/9.6

 ~35/10.1–10.3

  1. * Theoretical Mr/pI = theoretical molecular weight/isoelectric point, calculated from the amino acid sequence.
  2.  + Observed Mr/pI = observed molecular weight/isoelectric point, based on location following 2D electrophoresis.
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Veterinary Research

ISSN: 1297-9716