Immunoproteomic analysis of the serum IgG response to cell wall-associated proteins of Staphylococcus aureus strains belonging to CC97 and CC151

Drumm, Shauna D.; Cormican, Paul; Owens, Rebecca A.; Mitchell, Jennifer; Keane, Orla M.

doi:10.1186/s13567-023-01212-7

Veterinary Research

Table 2 List of MOK023 (CC97) candidate immunogenic CWA proteins detected by mass spectrometry.

From: Immunoproteomic analysis of the serum IgG response to cell wall-associated proteins of Staphylococcus aureus strains belonging to CC97 and CC151

Serum source	Spot number	Protein name	# Unique peptides	Predicted localisation	Theoretical Mr (kDa)/pI *	Observed Mr (kDa)/pI ⁺
Cow 604 (MOK023 infected)	1	Serine-aspartate repeat-containing protein E (SdrE)	50	Cell wall	126/4.46	~180/3.5–3.9
	1	Fibronectin binding protein B (FnbpB)	7	Cell wall	103/4.64	~180/3.5–3.9
	2	Serine-aspartate repeat-containing protein E (SdrE)	52	Cell wall	126/4.46	~180/4.1–4.5
	2	Fibronectin binding protein B (FnbpB)	11	Cell wall	103/4.64	~180/4.1–4.5
	3	Serine-aspartate repeat-containing protein E (SdrE)	59	Cell wall	126/4.46	~180/4.8–5.0
	3	Serine-aspartate repeat-containing protein D (SdrD)	14	Cell wall	148/4.39	~180/4.8–5.0
	4	Serine-aspartate repeat-containing protein E (SdrE)	34	Cell wall	126/4.46	~130/3.5–3.9
	4	Clumping factor B (ClfB)	19	Cell wall	95/4.2	~130/3.5–3.9
	5	Serine-aspartate repeat-containing protein E (SdrE)	53	Cell wall	126/4.46	~130/4.1–4.5
	5	Clumping factor B (ClfB)	26	Cell wall	95/4.2	~130/4.1–4.5
	6	Serine-aspartate repeat-containing protein E (SdrE)	50	Cell wall	126/4.46	~130/4.8–5.0
	6	Clumping factor B (ClfB)	28	Cell wall	95/4.2	~130/4.8–5.0
	7	Serine-aspartate repeat-containing protein E (SdrE)	18	Cell wall	126/4.46	~120/3.5–3.9
	7	Clumping factor B (ClfB)	15	Cell wall	95/4.2	~120/3.5–3.9
	8	Serine-aspartate repeat-containing protein E (SdrE)	31	Cell wall	126/4.46	~120/4.5–4.7
	8	Clumping factor B (ClfB)	13	Cell wall	95/4.2	~120/4.5–4.7
	9	Serine-aspartate repeat-containing protein E (SdrE)	26	Cell wall	126/4.46	~120/4.8–5.0
	9	Clumping factor B (ClfB)	22	Cell wall	95/4.2	~120/4.8–5.0
	10	Serine-aspartate repeat-containing protein E (SdrE)	8	Cell wall	126/4.46	~100/4.5–5.0
	10	Clumping factor B (ClfB)	3	Cell wall	95/4.2	~100/4.5–5.0
Cow 504 (MOK124 infected)	1	Serine-aspartate repeat-containing protein E (SdrE)	46	Cell wall	126/4.46	~180/3.9–3.98
	1	Serine-aspartate repeat-containing protein D (SdrD)	8	Cell wall	148/4.39
	2	Serine-aspartate repeat-containing protein E (SdrE)	46	Cell wall	126/4.46	~180/5.1–5.3
	3	Serine-aspartate repeat-containing protein E (SdrE)	18	Cell wall	126/4.46	~180/5.4–5.5
	4	Serine-aspartate repeat-containing protein E (SdrE)	47	Cell wall	126/4.46	~130/3.9–3.98
	4	Clumping factor B (ClfB)	23	Cell wall	95/4.2	~130/3.9–3.98
	5	Serine-aspartate repeat-containing protein E (SdrE)	21	Cell wall	126/4.46	~130/5.1–5.3
	5	Clumping factor B (ClfB)	11	Cell wall	95/4.2	~130/5.1–5.3
	6	Clumping factor B (ClfB)	12	Cell wall	95/4.2	~125/3.9–3.98
	6	Serine-aspartate repeat-containing protein E (SdrE)	8	Cell wall	126/4.46	~125/3.9–3.98
	8	Staphylococcal protein A (SpA)	3	Cell wall	41/5.53	~37/4.5–4.98
	9	Iron regulated surface determinant protein A (IsdA)	4	Cell wall	39/9.64	~35/3.4–3.6

^* Theoretical Mr/pI = theoretical molecular weight/isoelectric point, calculated from the amino acid sequence.
⁺ Observed Mr/pI = observed molecular weight/isoelectric point, based on location following 2D electrophoresis.

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ISSN: 1297-9716

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