Immunoproteomic analysis of the serum IgG response to cell wall-associated proteins of Staphylococcus aureus strains belonging to CC97 and CC151

Table 1 Plasmids used in this study.

Protein of interest	Plasmid description	S. aureus strain used for gene cloning	Protein domain or region cloned	Construct name	Antibiotic resistance marker	Final IPTG Concentration	References
ClfA	pQE30:ClfA N1N2N3	Newman	Complete A domain minus signal sequence AA 40-559	pCF40	Ampicillin	100 µM	[61]
ClfB	pQE30:ClfB N1N2N3	DU5966	Complete A domain minus signal sequence AA 44-542	pN123	Ampicillin	100 µM	[62]
FnbpB	pQE30:FnBPB N1N2N3	8325-4	Complete A domain minus signal sequence AA 37-480	pQE30::rFnBPB37–480	Ampicillin	100 µM	[63]
IsdA	pGEX2T TEV:IsdA	N315	Complete protein minus signal sequence and anchoring domain AA 48-316	GST-IsdA	Ampicillin	300 µM	[64]
SdrD	pSdrD-pRSETB-N-His	8325-4	Complete protein minus signal sequence and anchoring domain AA 53-1315	pRSETB-SdrD	Ampicillin	40 µM	[65]
SirA	pET28a:SirA_T37	N315	Complete protein minus signal sequence AA 37-330	SirA_T37	Kanamycin	300 µM	[66]

ISSN: 1297-9716