Figure 4

CD14⁺ PMNs in the lung produce more cytokines than their CD14⁻ counterparts. A, B Biaxial plots (A) from one representative experiment and bar charts (B) show the production of IL-17A, TNF-α, IFN-γ, IL-10, and IL-21 in CD14⁻ and CD14⁺ PMNs after stimulation with PMA and ionomycin. N = 6–12 samples in three independent experiments. Error bars indicate the mean ± SD. C Violin plots showing the RNA expression (log-normalized) of the indicated genes by CD14⁺ and CD14⁻ PMNs in the piglet lung after APP infection. D Bar plots show the frequencies of CD14⁺ and CD14⁻ PMNs in the piglet lung after APP infection. E GO and KEGG analyses show the main functions and signalling pathways of differentially expressed genes in CD14⁺ compared with CD14⁻ PMNs. All RNA-seq data were generated from one experiment with 3 samples per group. F The frequencies of CD14⁺ PMNs in the lungs after the indicated infection. N = 6 samples in three independent experiments.