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Figure 3 | Veterinary Research

Figure 3

From: MicroRNA miR-223 modulates NLRP3 and Keap1, mitigating lipopolysaccharide-induced inflammation and oxidative stress in bovine mammary epithelial cells and murine mammary glands

Figure 3

Bta-miR-223 directly targets NLRP3 and Keap1 on immortalized bMECs, respectively. A–C Homology of Bos taurus NLRP3 and Keap1 3’UTR and bta-miR-223. A The binding sites were between bta-miR-223 and the 3’UTR of NLRP3 and Keap1, respectively. B, C Data are plotted as the ratio between renilla luciferase activity and firefly luciferase activity. D–F Bta-miR-223 level in cultured bMECs challenged by LPS. D, E Intracellular levels of bta-miR-223 were quantified by FISH, scale bar, 100 μm, and F real-time PCR using individual SYBR Green miRNA assays. *p < 0.05 and **p < 0.01, differences compared to control group.

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Veterinary Research

ISSN: 1297-9716