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Figure 3 | Veterinary Research

Figure 3

From: Predominance of low pathogenic avian influenza virus H9N2 in the respiratory co-infections in broilers in Tunisia: a longitudinal field study, 2018–2020

Figure 3

Phylogenetic tree of NDV. The tree was constructed using F gene nucleotide sequences of Class II NDV. Sequences from this study were labelled with filled red triangle, previous NDV Tunisian sequences available in GenBank were labelled with black circle, 2019 NDV sequences from Tunisian backyard chickens were labelled with empty red triangle and reference sequences were unlabelled. The evolutionary history was inferred using the Maximum Likelihood method with Tamura 3-parameter model and 1000 bootstrap replications in MEGA X. All nucleotide positions containing gaps and missing data were eliminated. A total of 1675 positions were included in the final dataset. Only bootstrap values higher than 60 were conserved

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