Figure 4

Effects of miR-153 on IPEC-J2 cells. IPEC-J2 cells were transfected with miR-153 mimics or transfected with miR-153 inhibitor and then incubated with TsExos. Then, the assays were carried out. A Determination of the transfection efficiency of different concentrations of miR-153 mimics. B Changes in the transcription level of target genes after inhibiting TsExo-cargoed miR-153. C Western blotting was performed to detect the modulation of target genes via miR-153. The effect of miR-153 on cell proliferation through the CCK-8 assay at OD₄₅₀ values D, on cell damage by assessing the level of LDH at OD490 E, on cell permeability by detecting fluorescence intensity of FITC-dextran at 493-nm excitation and 517-nm emission wavelengths F, on cell oxidative stress detected by the fluorescence microplate reader at 488-nm excitation and 525-nm emission wavelengths G, and the fluorescence microscope at 20× PL FL magnification H. All assays were performed in triplicate, and data are presented as the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 indicate the significance levels of the comparisons between the mimic NC group and the mimic group, as well as between the inhibitor NC group and the inhibitor group.