Figure 2

Presence of haemolysis-associated genes within the genus of Actinobacillus sensu stricto. An ML tree (1000 ultrafast bootstraps) highlighting phenotypic (orange) haemolysis of new Actinobacillus strains in relation to haemolysis-associated genes embedded within all Actinobacillus sensu stricto genomes, including RTX protein hits across the Pasteurellaceae family. Colour code (yellow-magenta) represents amino acid identity of the identified proteins as compared to the NCBI RTX protein hits. Only hits with amino acid similarity above 60% and 80% coverage are shown, and RTX-like proteins were excluded. A complete overview can be found in Additional file 4.