Figure 1

Nsp9 interacts with H2BE. A Lysates of Marc-145 cells overexpressing Nsp9-EGFP and H2BE-Flag were subjected to reciprocal coimmunoprecipitation (co-IP) performed to detect protein interactions. B Lysates of Marc-145 cells overexpressing Nsp9-EGFP were subjected to co-IP and immunoblotting to detect any interaction between NSP9 and endogenous H2BE. C Effect of PEDV NSP9 overexpression on H2BE expression as detected by Western blotting. D IRX1 and H2BE mRNA expression levels were measured by qPCR. β-Actin was used as the internal control. E Western blot analysis was performed to assess the inhibition of IRX1 expression after Nsp9 overexpression in Marc-145 cells. F IRX1 and H2BE mRNA expression levels were measured by qPCR. β-Actin was used as the internal control. G Western blotting was performed to measure the expression of IRX1 and H2BE in Marc-145 cells under the indicated conditions. Marc-145 cells were transfected with si-IRX1 or NC-si, and the cells were collected 36 h later. H The protein levels of IRX1 and H2BE in si-IRX1-transfected MARC-145 cells were analysed by Western blotting. The results are representative of three independent experiments. The data are presented as the mean ± SD, n = 3 (*P < 0.05; **P < 0.01).