A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs

Table 1 The active ingredients of vaccines in this study

Vaccine^a	Adjuvant
Trial 1. Plasmid CpG adjuvant effect test
1. rPMT-NC^b + CpG	CpG (200 μg/mL)
2. rPMT-NC + w/o/w	w/o/w
3. Control (PBS)	–
Trial 2. rSly adjuvant dose-dependent test
4. rPMT-NC + w/o/w + rSly	rSly (100 μg/mL)
5. rPMT-NC + w/o/w + rSly	rSly (150 μg/mL)
6. Control (PBS)	–
Trial 3. Comparison with various adjuvants
7. rPMT-NC + w/o/w + rSly	rSly (100 μg/mL)
8. rPMT-NC + w/o/w + CpG	CpG (200 μg/mL)
9. rPMT-NC	–
10. Commercial vaccine^c	Al-gel
11. Control (PBS)	–

^aPig immunizaction vaccine with 2 mL by I.M.
^brPMT-NC (200 μg/mL).
^cIngredient: B. bronchiseptica (1 × 10⁹ CFU), P. multocida type A (1 × 10⁹ CFU).
P. multocida type D (1 × 10⁹ CFU), rsPMT/tox1 (20 μg), rsPMT/tox2 (20 μg), rsPMT/tox7 (20 μg), Adjuvant: Al-gel.

ISSN: 1297-9716