Bacterial chondronecrosis with osteomyelitis related Enterococcus cecorum isolates are genetically distinct from the commensal population and are more virulent in an embryo mortality model

Huang, Yue; Eeckhaut, Venessa; Goossens, Evy; Rasschaert, Geertrui; Van Erum, Johan; Roovers, Geert; Ducatelle, Richard; Antonissen, Gunther; Van Immerseel, Filip

doi:10.1186/s13567-023-01146-0

Veterinary Research

Table 1 Primer sequences used for PCR detection of potential E. cecorum virulence genes [15].

From: Bacterial chondronecrosis with osteomyelitis related Enterococcus cecorum isolates are genetically distinct from the commensal population and are more virulent in an embryo mortality model

Gene	Primers (5´-3´)	Amplicon(bp)	Gene function
ECS3_0190	F-TTGGCAGGAATTATCGATGTGATG	336	hypothetical protein
	R-CTAGTTCCTTTCTTCAACCTCGTC		hypothetical protein
ECS3_0193	F-ATGGAATTAAAGAAATATGCCAAAAGAATCC	222	hypothetical protein
	R-TTATTTCCATTGATATTCTGGATAACGATTT
ECS3_0196	F- ATGGAAAGAATAGATTTAACAAATAAGAAA	1083	dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)
	R-CTATTTATAAAACTCTTTATACCATTCTGCAA		dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)
ECS3_0199	F- GTGCTAATAAAAATAGATGATCCAGGACC	543	Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6) (wchA)
	R- TCAACTCTGCTTGCCCTCCTTTTTATTTA
ECS3_0200	F-TTGAAACGAATTTTAATAACTGGATTGAATAG	873	UDP-glucose 4-epimerase (EC 5.1.3.2)
	R-TCAAATTTCTGTCCTCTCAATTGATTCA		UDP-glucose 4-epimerase (EC 5.1.3.2)
ECS3_0201	F- TTGAAAAAGAAAGTCATGTTTTTAGTAAATCA	1095	Gylosyl transferase, cap 1E-like
	R-TTAAAAATTAATTTCAATCTCTACACAATATTTC TTTAC		Gylosyl transferase, cap 1E-like
ECS3_0202	F-ATGAATAAGTATGTAAGAGCAACATGT	564	Maltose O-acetyltransferase (EC 2.3.1.79)
	R-TTATTTAATAAACTGAATATTTCTAACACTTTTTCC		Maltose O-acetyltransferase (EC 2.3.1.79)
ECS3_0203	F-GTGGATAAGGAAGTAATAGTATTTATAGGA	1188	lipopolysaccharide biosynthesis RfbU-related protein
	R- TCACCTCATTTGTACATTTTTTATCAA		lipopolysaccharide biosynthesis RfbU-related protein
ECS3_0204	F-ATGAGAATTAATATTGCTTATGCTTGTGATG	930	Glycosyl transferase
	R-TTAATTAAAATACAAATATAACTTAACAAATAAAC TTTTGGGT		Glycosyl transferase
ECS3_0205	F-ATGAATATTAGGAAAACAATTCAAAATATGTTC	1422	Membrane protein involved in the export of O-antigen, teichoic acid lipoteichoic acids
	R-TTAATTTGAAATATTTTTCACTTTACAATTT AAAAGTCT
ECS3_0206	F-ATGAATTTTATTGTTATATTTCTTGTCTCGCTC	1197	hypothetical protein
	R-CTACCTAGAAATTTTTGAACTAAAATAAGCTAGC		hypothetical protein
ECS3_0211	F-ATGAATATGTTAGAAATGAAATATGAGCGC	318	FIG00410032: hypothetical protein
	R- TTAAAACCATTGTTCCACACCAGG		FIG00410032: hypothetical protein
ECS3_0212	F- ATGATTAAAGGGACCTCTGTTGCAAAAAG	561	hypothetical protein
	R-TTACCAAGAGATATTTTTATAATCTTTCTTGAAA		hypothetical protein
ECS3_0213	F- ATGGACAAACTTGTACCTGCATTTG	324	hypothetical protein
	R-TTAAAAGAATAATAACTCTGCCAAGTTCCTCT		hypothetical protein
ECS3_0662	F-ATGAATGGTATGCTTCATGATTTGAAA	804	Lipoate synthase
	R-TCATTTGCTCGCTCCACCATCGGCTAAT		Lipoate synthase
ECS3_2294	F-AACGATTTTCAAAGAGAACTTCCTCAG	936	Glycosyltransferase
	R-TACGCAACAATTTAAGAAAAAATGCCTAAG		Glycosyltransferase
ECS3_2299	F-ATGAGTTCGATTGGAGTTTTTAATTCTGTC	1860	epaP-like (hypothetical protein)
	R-GGTTGGCATTCAGGTAAAAATTAGA		epaP-like (hypothetical protein)
ECS3_2316	F-ATGAGTTCGATTGGAGTTTTTAATTCTGTC	1146	FIG00629489: hypothetical protein LPTXG3 domain
	R-ACAAATAGTCAGCTTTTTATTGATAAAATT		FIG00629489: hypothetical protein LPTXG3 domain

Amplicon sizes and (hypothetical) virulence gene functions are listed.

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ISSN: 1297-9716

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