Figure 2

The impacts of OAg length on bacterial colonization in mice, cell permeability and antibiotic resistance. Colonization of ATCC14028, ST03 and ST04 in mice intestines (A), spleens (B), and livers (C). D Membrane permeability of ATCC14028, ST03 and ST04. The study was performed by recording the fluorescence of Ethidium influx into wzz substitution mutant strains at 600 nm with excitation at 545 nm. Blank control is ATCC14028 suspended in 50 mM potassium phosphate buffer without Ethidium bromide, PC control is ATCC14028 treated with 75% ethanol for 1 h. E Antibiotic sensitivity assay. The inhibitory zone diameter for each strain was measured. Error bars represent the standard deviation of 3 replicate samples.