Table 1 Primers in this study
Primer name | Sequence (5ʹ–3ʹ) |
|---|---|
Primers used for detection of transcription of genes in STEC | |
GAPDH F | TGGTCACCAGGGCTGCTT |
GAPDH R | CATGTAGTGGAGGTCAATGAAGG |
IL-8 F | ATTCCACACCTTTCCACCCC |
IL-8 R | CCACTTTTCCTTGGGGTCCA |
IL-6 F | AAATGTCGAGGCTGTGCAGA |
IL-6 R | TCCACTCGTTCTGTGACTGC |
TNF-α F | GCACTGAGAGCATGATCCGA |
TNF-α R | GAAGGAGAAGAGGCTGAGGC |
NOD2 F | CGTCTGCAAGGCTCTTTACTTG |
NOD2 R | GCCGTCGGTCAATTTGTTGT |
Vimentin F | CAGATCCAGGAACAGCACGT |
Vimentin R | GAGAGGTCGGCAAACTTGGA |
Primers used for detection of transcription of mice genes | |
mGAPDH F | GGTGGAGCCAAAAGGGTCAT |
mGAPDH R | GGGGGCTAAGCAGTTGGTG |
mIL-6 F | TGGTCTTCTGGAGTACCATAGC |
mIL-6 R | CTGTGACTCCAGCTTATCTCTTG |
mKC F | ACTCAAGAATGGTCGCGAGG |
mKC R | ACTTGGGGACACCTTTTAGCA |
mNOD2 F | TCTGGAGGTTTGGCTTCGAG |
mNOD2 R | ACAACAAGAGTCTGGCGTCC |
mIL-1β F | ACTCAACTGTGAAATGCCACCT |
mIL-1β R | TGTGCTGCTGCGAGATTTGA |
mTNFα F | ACTGAACTTCGGGGTGATCG |
mTNFα R | TGAGGGTCTGGGCCATAGAA |