Figure 2

The deficiency of vimentin prevents the recruitment of neutrophils in the airway. A Immunohistochemistry of the lung of PBS- or SS2-infected mice (n = 3 mice/group) at 24 hpi using the neutrophil marker Ly-6G. Red arrows indicate the neutrophils. Scale bar, 100 μm. B Neutrophil numbers in BALF of infected mice were quantified by FACS. Anti-Mouse CD11b and APC Anti-Mouse Ly-6G (Gr-1) antibodies were used to label neutrophils. C–E RT-qPCR detected the transcription of proinflammatory cytokines IL-6 (C), TNF-α (D), and IL-8 (E) in STEC and VIM KO STEC infected with SS2 (MOI 50) for 2 h. Data are representative or are presented as the mean ± SD. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.