Figure 7

The 3CD protein reduces RIG-I protein through the proteasome pathway. Cells in 12-well plates were co-transfected with 3CD-HA (0.6 µg) or empty plasmid and RIG-I (N-terminal)-Flag (0.4 µg). At 24 h post-transfection, the broad-spectrum caspase inhibitor Z-VAD-FMK (50 µM), proteasome inhibitor MG132 (20 µM), autophagy inhibitor 3-MA (10 mM), and autophagy-lysosome inhibitor NH₄Cl (10 mM) were added to the cell culture medium, while a DMSO negative control group was set up and treated for 12 h. Cell samples were collected after 12 h of treatment, and immunoblotting experiments were performed in the presence or absence of these inhibitors. The expression level of RIG-I was detected. A The effect of MG132 on RIG-I protein degradation. B The effect of 3-MA on RIG-I protein degradation. C The effect of Z-VAD-FMK on RIG-I protein degradation. D The effect of NH₄Cl on RIG-I protein degradation.