Figure 7

Biofilm formation ability. The biofilm formation ability of each strain at different time points determined by crystal violet staining A; The biofilm formation ability of each strain at different time points determined by viable count method B; The biofilm formation ability of each strain observed by LSCM C; Live bacteria are labeled with cyto9 dye, dead bacteria are labeled with PI dye, and merge means the result of mixing both fluorescences. The biofilm volume of each strain observed by LSCM D; The biofilm residual amount of each strain after proteinase K treatment E; The 3D construction map of biofilm of each strain observed by LSCM F (a: LM, b: LI, c: LIΔilo, d: LIΔilo::hly); The biofilm thickness of each strain observed by LSCM G; Micro-images of each strains’ biofilm on the 3rd day on glass slides taken by SEM. The micro-images were taken at ×1000, ×5000 magnifications H; ^** P < 0.01, vs. LI; ^# P < 0.05, ^## P < 0.01, ^### P < 0.001, vs. LIΔilo; n = 3.