Figure 3
chTERT gene was successfully overexpressed in chickens. A Western blot analysis of the expression of chTERT-FLAG fusion protein in various tissues after transfection in vivo. B The chTERT-FLAG protein expressed in 293 T cells in vitro and its control (mock) were used as antigens, and chicken serum was used as an antibody for Western blot analysis. The results showed that when chicken serum overexpressing chTERT was used as an antibody, a band of approximately 150 kD could be successfully detected, which was consistent with the positive control (FLAG antibody) but not detected in the control group, indicating that chTERT was successfully overexpressed in vivo. C Chicken blood cell DNA was extracted, and the eGFP gene was amplified by PCR. Lanes 1–3: chicken blood DNA of the chTERT overexpression group; lanes 4–6: chicken blood DNA in the control group; lane 7: positive control; lane 8: negative control. The results showed that the eGFP gene with a size of approximately 720 bp could be successfully amplified from chicken blood DNA, indicating that the in vivo transfection was successful. Since chTERT and eGFP genes were fused and overexpressed in the experimental group, and eGFP gene was only carried in the control group, eGFP gene could also be amplified in the blood DNA even in the control group without overexpression of chTERT.