Table 1 Sequences of DNA primers
From: Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis
Name | Primer Sequence (5′-3′) |
|---|---|
Primers for CDS Cloning | |
 SOCS5-CDS-F | TAGCGTTTAAACTTAAGCTTATGGATAAAGTGGGAAAGATGT |
 SOCS5-CDS-R | CACAGTGGCGGCCGCTCGAGTTACTTTGTTTTTATAGGTTCCC |
 SOCS5 3’UTR-F | GGCTCGAG AGATGCTGCCTGCTGTTAATC |
 SOCS5 3’UTR-R | ATGCGGCCGCGCAGGTTATCCCTGCTTCTTG |
Primers for RT-qPCR | |
 RT-gga-miR-5 S | AACTGGTGTCGTGGAGTCGGC |
 gga-5s-rRNA-F | CCATACCACCCTGGAAACGC |
 gga-5s-rRNA-R | TACTAACCGAGCCCGACCCT |
 RT-gga-miR-365-3p | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGATAAGGAT |
 gga-miR-365-3p-F | GGTAGGTAATGCCCCTAAAAATCC |
 gga-miR-365-3p-R | ACTGGTGTCGTGGAGTCGGC |
 GAPDH-F | CCTCTCTGGCAAAGTCCAAG |
 GAPDH-R | TTGATGTTGCTGGGGTCACG |
 SOCS5-F | GGCACTACCAGTACAAAAGCATC |
 SOCS5-R | CAAGGACCTGCGACTCGAAC |
 STAT1-F | CTTGATGCTGGGAGAGGAGT |
 STAT1-R | TGAGGGAGAGAGAGCGAAAG |
 STAT2-F | AGCAAAACTGTCCTGGTTGG |
 STAT2-R | GACCCTCATTGGTGCCTCT |
 STAT3-F | AAGCGTGGTCTCAGCATTGA |
 STAT3-R | CCAGCCAGACCCAGAAAGAG |
 STAT4-F | TGAAAGCAATCTGGGTGGA |
 STAT4-R | TCGCAGTATGTCAGCAAAGG |
 STAT5-F | TTGACCTGGACGACACCAT |
 STAT5-R | GACACAAACACGGCACAGTC |
 STAT6-F | CCTGAAGAGCTACTGGTCGG |
 BCL2-F | AAGCAAGCGTGACAAC |
 BCL2-R | ATCATAGGCTGCACATAC |
 BCLXL-F | CGGCTCATCATCCAGTCC |