Figure 2

The effect of gga-miR-365-3p CP- cells. CP- cells were transfected with siRNA for 24 h and then infected with MG-HS (A). qPCR was used to detect the mRNA expression of pMGA1.2. GAPDH was used as the internal quantitative control gene (B, C). CP cells were transfected with gga-miR-365-3p 50 nM mimics, 100 nM inhibitors, or their respective control and infected with 100 µL of MG-HS (1 × 10¹⁰ CCU/mL). Then CCK-8 kit was used to detect cell proliferation at different times (B). After 48 h of treatment, the CP- cells were stained with Annexin V– PI, and analyzed by flow cytometer. Apoptosis cell ratio is shown in (C).