Figure 6

FABP4 is involved in LD increases during S. uberis infection. RAW264.7 cells were treated with 10 µM C75 for 24 h to inhibit fatty acid synthesis and then incubated with 360 µM oleic acid for 24 h. S. uberis in mid-exponential phase (MOI = 10) was added for 3 h at 37 ℃. A Relative Fabp4 mRNA expression. B The protein expression levels of FABP4 with or without C75 or oleic acid during S. uberis infection were determined by Western blots in RAW264.7 cells. For quantitative analysis, bands were evaluated densitometrically with ImageJ analyser software normalized for β-actin density. C Immunofluorescence and LD staining were performed by FABP4 (red) and BODIPY (green) in the control group and stimulated S. uberis groups as well as the chemical pretreatment groups. Images are representative of n = 10 samples/group. Scale bar, 50 µm. Experiments A–G were repeated 3 times. Data are presented as the means ± SEMs (n = 6, unless otherwise indicated). *(P < 0.05) = significantly different between the indicated groups by one-way ANOVA.