Figure 6From: Genotyping and biofilm formation of Mycoplasma hyopneumoniae and their association with virulence Biofilm formation ability of M. hyopneumoniae strains. A–F Biofilms formed by strain NJ, which were stained with crystal violet and observed under a microscope after A 1 day, B 2 days, C 3 days, D 4 days, and E 5 days of incubation. F negative control. G, H Comparison of biofilms formed by seven M. hyopneumoniae strains. Biofilm formation ability of strains was determined and identified by crystal violet staining after 4 days of incubation. Bars represent mean ± SD for three independent replicate experiments. KM2 represents control (uninoculated broth).Back to article page