Figure 7
From: Long noncoding RNA IRF1-AS is associated with peste des petits ruminants infection
Identification of PPRV-induced IRF1-AS. A Schematic representation of the IRF1-AS and IRF1 locus in the Capra hircus genome. B–D EECs were infected with PPRV at an MOI of 3 for the indicated times, and the transcription levels of IRF1-AS (B), IRF1 (C) as well as the protein levels of IRF1 (D), were measured by quantitative RT-PCR and Western blot assay, respectively. E The relative quantification of IRF1 protein levels compared to β-actin protein levels was determined by densitometry. β-actin was used as a loading control in Western blot analysis. The data represent the mean ± SD of three independent experiments. P values were calculated using Student’s t test. An asterisk indicates a comparison with the indicated control. n.s., no significant; **P < 0.01; ***P < 0.001.