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Table 1 Summary of species identification results obtained by MALDI-TOF MS and gap PCR–RFLP on the 261 isolates evaluated in this study

From: Species identification by MALDI-TOF MS and gap PCR–RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis

Species ID by MALDI-TOF MSa

N. of isolatesb

Log score

AR with gap PCR-RFLPf

 ≥ 2.0c

1.7–1.99d

 < 1.7 (no ID)e

Staphylococci

Staphylococcus epidermidis

59 (28.9%)

55 (93.2%)

4 (6.8%)

–

59 (100%)g

Staphylococcus chromogenes

57 (27.9%)

53 (93.0%)

4 (7.0%)

–

57 (100%)g

Staphylococcus haemolyticus

32 (15.7%)

18 (56.3%)

14 (43.7%)

–

32 (100%)g

Staphylococcus caprae

13 (6.4%)

10 (76.9%)

3 (23.1%)

–

13 (100%)g

Staphylococcus simulans

13 (6.4%)

13 (100%)

–

–

13 (100%)g

Staphylococcus microti

6 (2.9%)

3 (50%)

3 (50%)

–

0 (0%)

Staphylococcus xylosus

4 (2.0%)8

2 (50%)

2 (50%)

–

2 (50%)g

Staphylococcus equorum

3 (1.5%)

3 (100%)

–

–

3 (100%)g

Staphylococcus petrasii

3 (1.5%)

3 (100%)

–

–

0 (0%)

Staphylococcus warneri

3 (1.5%)

2 (66.7%)

1 (33.3%)

–

3 (100%)g

Staphylococcus sciurih

2 (1.0%)

2 (100%)

–

–

0 (0%)g

Staphylococcus arlettae

1 (0.5%)

–

1 (100%)

–

1 (100%)g

Staphylococcus capitis

1 (0.5%)

1 (100%)

–

–

1 (100%)g

Staphylococcus cohnii

1 (0.5%)

–

1 (100%)

 

0 (0%)

Staphylococcus lentush

1 (0.5%)9

–

1 (100%)

–

0 (0%)

Staphylococcus pseudintermedius

1 (0.5%)

–

1 (100%)

–

1 (100%)g

Staphylococcus succinus

1 (0.5%)

–

1 (100%)

–

0 (0%)

Unidentified

3 (1.5%)

–

–

3 (100%)

–

Total staphylococci

204

165 (80.9%)

36 (17.6%)

3 (1.5%)

185 (90.7%)

Streptococci

Streptococcus uberis

51 (89.5%)

48 (94.1%)

3 (5.9%)

–

51 (100%)g

Streptococcus dysgalactiae910

2 (3.5%)

2 (100%)

–

–

2 (100%)g

Streptococcus parauberis

2 (3.5%)

2 (100%)

–

–

2 (100%)k

Streptococcus gallolyticus

1 (1.8%)

1 (100%)

–

–

1 (100%)g

Streptococcus suis

1 (1.8%)

–

1 (100%)

–

0 (0%)g

Unidentified

0 (1.8%)

–

–

–

–

Total streptococci

57

53 (93.0%)

4 (7.0%)

0 (0%)

56 (98.2%)

  1. aBased on the Bruker MALDI Biotyper System Compass® Library Revision H (2021), covering 3893 species/entries.
  2. bPercent data represent the proportion of a given species isolated among all staphylococci or streptococci (within column).
  3. cPercent data represent the proportion of isolates identified with MALDI log scores ≥ 2.0 among all isolates of the same species.
  4. dPercent data represent the proportion of isolates identified with MALDI log scores 1.7–1.99 among all isolates of the same species.
  5. ePercent data represent the proportion of isolates with MALDI log scores < 1.7 among all isolates of the same species and no identification possible (no ID).
  6. fNumber of isolates identified as the same species by MALDI-TOF MS and gap PCR–RFLP. Percent data represent the proportion of isolates identified as the same species (Agreement Rate, AR).
  7. gThe reference strain for this species was included in the gap PCR–RFLP identification panel (please see the materials and methods section for the reference isolate list).
  8. hThese species have been reclassified in the Mammaliicoccus genus [6].
  9. iFor one isolate, the first round of MALDI-TOF MS identification was not successful and it was repeated.
  10. jStreptococcus dysgalactiae and Streptococcus canis cannot be resolved by MALDI-TOF MS.
  11. kBoth assigned by gap gene amplicon sequencing and alignment with sequences in the GenBank database using the Basic Local Alignment Search Tool (BLAST). Details are reported in Additional file 7.
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Veterinary Research

ISSN: 1297-9716