Figure 7

miR-126-5p inhibits chicken antiviral immune responses by targeting TRAF3. A miR-126-5p and TRAF3 binding site prediction. B qPCR detection of the expression of TRAF3 after over-expression of miR-126-5p. C qPCR detection of the expression of TRAF3 after inhibition of miR-126-5p. qPCR analysis of the expression of TRAF3 in DF1 cells that were overexpressed (D) or inhibited (E) miR-126-5p 12 h, infected with NDV for 24 h at an MOI of 0.01, and then treated with actinomycin D (ActD; 10 g/mL) for the indicated times. F The TRAF3 3'UTR containing the miR-126-5p binding sequence or mutant sequence genes was cloned into the pmiR-GLO vector as shown. G HEK293T cells were transfected with pmiR-TRAF3-WT-GLO or mutant pmiR-TRAF3-mutant-GLO along with miR-126-5p mimics or NC, lysed after 24 h, and subjected to luciferase assay. The data are expressed as the mean ± SD; n = 3. *p < 0.05, **p < 0.01.