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Figure 6 | Veterinary Research

Figure 6

From: Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP

Figure 6

Testing the biofilm reformation ability and antibiotic sensitivity of dispersed cells. CRAMP was used to disperse the biofilm and was preformed for 3 days, and the dispersed cells were collected for testing, with planktonic cells as a control. A represents the biofilm reformation capacity of dispersed cells and planktonic cells within 14 h, and the biomass was detected by crystal violet staining. B represents the antibiotic sensitivity of dispersion cells and planktonic cells, the Y-axis represents the survival rate of dispersion cells and planktonic cells, and the X-axis represents the exposure time to drugs. The dotted line represents planktonic cells, and the solid line represents dispersed cells. Black filled triangle represents planktonic cells that were treated with CRAMP (2 MBCs, 62.5 µg/mL); Black filled circle represents planktonic cells that were treated with ciprofloxacin (2 MBCs, 0.125 µg/mL); Black filled square represents dispersed cells that were treated with CRAMP (2 MBCs, 62.5 µg/mL); Black filled inverted triangle represents dispersed cells that were treated with ciprofloxacin (2 MBCs, 0.125 µg/mL). × represents dispersed cells that were treated with CRAMP (8 MBCs, 250 µg/mL); Black filled star represents dispersed cells that were treated with ciprofloxacin (8 MBCs, 0.5 µg/mL). An unpaired t test (two-tailed) was used to measure statistical significance. *P < 0.05, **P < 0.01.

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