Figure 2
From: Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP
Confocal laser scanning microscopy of the eradication of PAO1 biofilms treated with CRAMP. The bacterial suspension was cultured in a chamber coverglass for 3 days, and the biofilm was treated with CRAMP for 1 h. Staining with SYTO 9 (green, live) and propidium iodide (red, dead) was performed under objective 20× and 63× oil by CLSM. The orthogonal views of Z-stacks and 3D images are presented (ZEN 2.3 Viewer v2.3.69.1000 software). Image processing was carried out using BiofilmQ software. A and E represent the orthogonal views and 3D biofilm representation in the objective of 20× in the control group. B and F represent the orthogonal views and 3D biofilm representation of CRAMP in the objective of 20×. C and G represent the orthogonal views and 3D biofilm representation of the objective of 63× in the control group. D and H represent the orthogonal views and 3D biofilm representation of CRAMP in the objective of 63×. I represents the number of biofilms treated with CRAMP. J represents the biofilm base area treated with CRAMP. K represents the volume of biofilms treated with CRAMP. L represents the fluorescence intensity of biofilms treated with CRAMP. M represents the ratio of fluorescence intensity to biofilm base area treated with CRAMP. An unpaired t test (two-tailed) was used to measure statistical significance. * P < 0.05, ** P < 0.01, **** P < 0.0001 compared with the control group.