Figure 3

NLRP6 mediates expression of inflammatory cytokines in macrophages infected with P. multocida. WT and Nlrp6^−/− mice were intranasally infected with 20 µL P. multocida (1000 CFU) and the same volume of sterilized PBS were used as the control. Bronchoalveolar lavage fluid (BALF) and peripheral serum were collected after 48 h infection (n = 5–8). ELISA was used to detect the levels of IL-1β (A), IL-6 (B), TNF-α (C) in BALF and IL-1β (D), IL-6 (E) and TNF-α (F) in peripheral serum (n = 5–8). Macrophages from WT or Nlrp6^−/− mice were infected with P. multocida at an MOI of 1 for 9 h. Then 100 μg/mL ciprofloxacin was added for an additional 15 h incubation. After 24 h infection, the supernatants were collected, and IL-1β (G), IL-6 (H) and TNF-α (I) were detected by ELISA. The student’s t-test was used to analyze the statistical differences for comparisons between two groups. Statistical significance was determined as the p value, *P < 0.05, **P < 0.01, ns: no significance.