Figure 2

NLRP6 affects the formation of NET. Neutrophils from WT or Nlrp6^−/− mice were infected with P. multocida at a MOI of 1 for 3 h. The supernatants (Sup) and cell lysates (Lys) were collected after infection. Cit H3 protein expression was detected by western blot analysis and immunofluorescent staining. A representative image of Cit H3 protein expression is shown (A) The number of neutrophils with NET was quantified (B). Cit H3 protein (red) and DAPI (blue) were observed through fluorescence microscopy (C). The white arrow indicates the co-localization of Cit H3 and DAPI. Image J was used for quantitative analysis. The results are representative of three independent experiments. The student t-test was used to analyze the statistical differences for comparisons between two groups. Statistical significance was determined as p value, *P < 0.05, **P < 0.01, ns: no significance.